Anesthetic effects of Cuminum cyminum essential oil and 2-phenoxyethanol on zebrafish (Danio rario)

investigation, the efficacy of two anesthetic agents Cuminum cyminum essential oils (EOs) and 2- in different dosages were compared in zebrafish, Danio Fish (1.3 ± 0.2 g) were anesthetized with different concentration of C. (0.12, 0.16, 0.20, 0.24, 0.32 and 0.40 ml/l) and 2- (0.35, 0.40, 0.45, 0.50, 0.55 and 0.60 ml/l) by After that, the anesthesia was induced and the biometric measurements were completed, fish were transferred to anesthetic-free aquaria to allow for recovery. The lowest effective concentrations based on the efficacy criteria of complete anesthesia induction within 180s and recovery within 300s were determined to be 0.24 ml/l (induction: 157.6±55.3s and recovery time: 133.3±14.2s) for C. and 0.35 ml/l (induction: 126.6±23.6s and recovery time: 124.6±31.5s) for 2-. An inverse exponential relationship was observed between concentrations of anesthetics (C. and 2-) and induction time. This study shows the high potential of C. for fish anesthesia, and the C. different concentrations (0.24-0.40 ml/l) seem to be suitable for anesthetization of